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KMID : 1098420050130030093
Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 3 p.93 ~ p.100
Antifungal and Antioxidant Activities of Extracts from Leaves and Flowers of Camellia japonica L
Lee Sook-Young

Hwang Eun-Ju
Kim Ji-Hey
Choi Young-Bok
Lim Chae-Young
Kim Sun-Min
Abstract
This research was performed to investigate the possibilities of industrial usage of camellia (Camellia japonica L.) by examining the antioxidant and antimicrobial effects of methanol extract with different sections. Content of total phenolics, DPPH radical scavenging activities and antibacterial activity of young leaf, mature leaf, flower bud, flower, bark, and seed of camellia were compared in vitro experimental models. Total phenolics was contained the higher in young leaf (74.62 mg), flower bud (65.02 mg) and flower (62.42 mg) but less than 20.95 mg per 100 g of dry weight in other parts of Camellia japonica L. And effects of antioxidant measured by DPPH radical scavenger activity (RC_{50}, reduce concentration 50%), was shown higher 7.16{sim}18.14;{mu}g/m{ell} in methanol extract of young leaf, flower bud and flower than 61.23;{mu}g/m{ell} of BHT as a chemical oxidant. Also, the antimicrobial activity of Camellia japonica L. extracts determined using a paper disc method against food-borne pathogen and food spoilage bacteria, the young leaves extracts showed the most active antimicrobial activity against 7 kinds of harmful microorganisms. Flower bud extracts showed the highest antibacterial activity against P. aeruginosa and Enterobacter spp. C1036. In addition, the minimum inhibitory concentration (MIC) of young leaf extract against B. subtillis,S. fradiae,S. aureus,E. coli,P. aeruginosa, Enterobacter spp. C1036, and S. typhimurium were revealed 1 to 15 {mu}g/m{ell}. As a result, antimicrobial activity of camellia extracts was shown higher gram positive bacteria than gram negative bacteria.
KEYWORD
Camellia japonica, phenolic compound, DPPH radical, antibacterial activity, MIC
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